How is Southern blotting used in forensics?įorensic laboratories used southern blotting method to detect the smallest quantity of DNA, especially in the case of rape, thieves, or identification of parenthood. It helps detect cancer and genetic diseases like sickle cell mutation and monoclonal leukemia.It has the ability to identify a single gene in a pool of DNA fragments.
#Southern blotting technique series#
There are a series of steps that need to be strictly followed when performing Southern blotting.
(5, 6, and 7) What are the steps in Southern blotting? The DNA fragments are identified using a labeled probe hybridization. Basically, Southern blotting separates DNA fragments by gel electrophoresis. It is a hybridization method for identifying the size of DNA from a mixture of other similar molecules. Southern blotting is a restriction fragment length polymorphism. What is the principle of Southern blotting? The amount of DNA needed varies depending on the size and specific activity of the probe. How much DNA is needed for Southern blotting? They will be transferred to a membrane, hybridize, washed, and lastly, detecting the labeled DNA band. The Southern blotting method is a classic technique that separates DNA fragments according to their size through electrophoresis. A southern blot analysis reveals the following:
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